PROVEPEP (now Provepharm Solutions) successfully synthesized IL-10 under GMP (160 mer containing 2 disulfide bridges), obtained by SPPS using our SEA technology.
IL-10 is an anti-inflammatory cytokine produced primarily by macrophages, regulatory T cells, and epithelial cells (1). IL-10 has pleiotropic effects in immunoregulation and inflammation, inhibiting the production of multiple, diverse inflammatory mediators (e.g., IL-12, MHC class II antigens and costimulatory molecules) from activated macrophages and dendritic cells—a process requiring STAT3 activation. It also down regulates the expression of Th1 cytokines (such as IFN-γ, TNF, IL-1β, and IL-6) and impairs secondary CD8+ T cell responses. More generally, IL-10 enhances B cell survival, proliferation, and antibody production. IL-10 deficiency in mice suggests the function of this cytokine as an essential immunoregulator in the intestinal tract, and mutations in IL-10 are also associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.